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Item #:
127089938
50kDa Ultra Centrifugal Filter Unit, 0.5mL, Regenerated Cellulose RC Membrane, Snap Caps (24/PK)
Item #:
127089938
USD 81
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Fast ultrafiltration with high concentration factors and a protein recovery rate >90%.
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What Stands Out
High Capacity
Each filter unit holds 0.5mL, accommodating larger sample volumes for efficient separation and concentration, catering to lab professionals needing robust filtration solutions.
Durable Design
Constructed from polypropylene with snap caps, ensuring leak resistance and longevity, making it ideal for repetitive laboratory use and enhancing reliability in critical experiments.
RC Membrane
Utilizing a 300kDa RC membrane, these filters achieve superior protein retention and filtration efficiency, effectively addressing volume and concentration challenges in various research applications.
Product Details
| Item Weight | 1.5 lbs (680 grams) |
Who Should Buy?
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Research Laboratories
Essential for researchers needing reliable sample concentration and purification during biochemical and molecular experiments.
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Biotechnology Firms
Ideal for biotechnology companies focused on protein purification or large-scale biochemical assays requiring high efficiency.
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Clinical Applications
Useful in clinical labs needing to filter and concentrate biological samples for diagnostic testing and analysis.
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Home Use
Not suitable for casual users or home experiments due to the complexity and specificity of the filtration process.
Product Description
50kDa Ultra Centrifugal Filter Unit, 0.5mL, Regenerated Cellulose RC Membrane, Snap Caps (24/PK)
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Customer Questions & Answers
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Question:
What is an ultrafiltration centrifugal filter and how does it work?
Answer: An ultrafiltration centrifugal filter is a specialized device used to separate particles in a fluid based on their size. It employs a regenerated cellulose (RC) membrane with a molecular weight cut-off of 300kDa, allowing smaller solutes to pass through while retaining larger molecules. The centrifugal force applied during the process enhances the filtration efficiency, making it ideal for concentrating proteins, enzymes, or nucleic acids in biological research. This type of filter is commonly used in laboratories for applications like sample preparation, protein purification, and concentrating biological fluids. -
Question:
What are the applications of using 0.5mL centrifuge tube units?
Answer: The 0.5mL centrifuge tube units are designed for minimal sample volume, making them ideal for applications requiring precision with small quantities. They are frequently used in molecular biology for DNA/RNA extraction, protein concentration, and separating cellular components. Additionally, their small size reduces reagent waste and allows for higher throughput in assays. These tubes are particularly useful in research where sample availability is limited, providing scientists the ability to work effectively without compromising on quality or accuracy. -
Question:
Are the filters reusable or disposable?
Answer: The 24/pk 0.5mL ultrafiltration centrifugal filters are intended for single-use to ensure sample integrity and avoid cross-contamination. Each filter is designed to maintain optimal performance by providing a clean environment for your samples. While it may seem cost-effective to reuse filters, doing so can lead to inaccurate results due to residues or changes in the membrane properties. For precise laboratory experiments and consistent results, it is best to use a new filter for each sample run. -
Question:
What is the maximum centrifugal force that can be applied to these filters?
Answer: The ultrafiltration centrifugal filters typically can withstand centrifugal forces up to 16,000 x g. This high centrifugal force is essential to efficiently drive the filtration process, ensuring that the desired solutes pass through while larger particles are retained. It's crucial to adhere to the manufacturer's guidelines for centrifugation speeds to avoid damaging the filter membrane and compromising your results. In practice, users might employ these filters in centrifuges equipped with appropriate rotors designed for high-speed applications. -
Question:
Can these filters be used for any type of sample, including viscous liquids?
Answer: The ultrafiltration centrifugal filters are versatile and can be used for various sample types, including aqueous solutions, organic solvents, and viscous liquids. However, for particularly viscous samples, pre-dilution may be necessary to avoid clogging the membrane. The 300kDa cut-off membrane efficiently separates proteins and other macromolecules in different sample matrices. Applications are broad, from preparing cell lysates to concentrating protein solutions, hence making it suitable for a range of biochemistry and molecular biology experiments. -
Question:
What is the provided membrane material and its benefits?
Answer: The filters utilize a regenerated cellulose (RC) membrane, known for its high permeability and biocompatibility. This material provides excellent flux rates and minimal protein binding, allowing for efficient concentration and purification processes without significant loss of your target molecules. The biocompatible nature of RC membranes makes them suitable for sensitive biological samples, ensuring integrity throughout the filtration process. Researchers often prefer RC for its robustness in a variety of applications, including purification and concentration of proteins and nucleic acids. -
Question:
What should I consider when storing these filters?
Answer: When storing the ultrafiltration filters, it's important to keep them in a dry environment away from direct sunlight to preserve the integrity of the membrane. It is recommended to store them in their original packaging until use to prevent contamination. Additionally, avoid exposing the filters to extreme temperatures, as this could affect their performance. Proper storage helps maintain their efficacy for high-quality filtering when you are ready to conduct your experiments. Storing them correctly ensures that you achieve reliable results from your research work. -
Question:
Is there a specific protocol for using these filters in experiments?
Answer: Yes, following a specific protocol when using ultrafiltration centrifugal filters is essential for optimal results. Generally, you should start by preparing your sample and loading it into the filter's reservoir without overfilling. After securing the cap, place the filter in a centrifuge and select the appropriate speed and time based on the recommended guidelines. Post-centrifugation, collect the filtrate and analyze as required. Adhering to a consistent protocol enhances reproducibility and helps in obtaining reliable experimental outcomes. -
Question:
Where can I buy 24/pk 0.5mL 300kDa Ultrafiltration Centrifugal Filter Centrifuge Tube Unit with Regenerated Cellulose RC Membrane in Palau?
Answer: You can purchase the 24/pk 0.5mL 300kDa Ultrafiltration Centrifugal Filter Centrifuge Tube Unit with Regenerated Cellulose RC Membrane from Ubuy. Ubuy offers a variety of laboratory supplies, ensuring you have access to high-quality filters for your research needs. With a user-friendly platform, it's convenient to find the specific products you need for your experiments, all in one place. -
Question:
How do these filters compare to other filtration methods?
Answer: Ultrafiltration centrifugal filters offer several advantages over traditional filtration methods such as gravity filtration or vacuum filtration. They can efficiently separate molecules based on size without additional assistance like applying pressure. This method can yield quicker results, particularly with smaller sample volumes. Compared to other laboratory techniques, ultrafiltration is less labor-intensive and can often provide higher purity levels, making it ideal for sensitive applications. Many researchers prefer these filters for their convenience and efficiency in purifying and concentrating samples with minimal intervention.
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USD 81
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Features & Benefits
- Experience rapid ultrafiltration with exceptional concentration factors.
- Enjoy over 90% protein recovery rate for effective results.
- Quick processing time between 10-30 minutes.
- Compatible with a wide range of chemicals.
- Available in various molecular weight cut-offs (MWCO) for precise filtration.
- Conforms to ISO 9001:2015 standards, ensuring top-quality performance.
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